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| Lunardi,Lígia Uno; Guembarovski,Roberta Losi; Hanai,Luiz Ricardo; Cristiano,Valderi; Vieira,Maria Lucia Carneiro; Sartori,Daniele; Fungaro,Maria Helena Pelegrinelli. |
| Ochratoxin A is a mycotoxin produced by some fungi species. Among them, Aspergillus carbonarius is considered a powerful producer. Genes involved in the ochratoxin A biosynthesis pathway have been identified in some producer species. However, there are few studies that purpose to identify these genes in A. carbonarius. The use of insertion mutants to identify genes associated with certain properties has been increased in the literature. In this work, the region of T-DNA integration was investigated in one A. carbonarius ochratoxin-defective mutant previously obtained by Agrobacterium tumefaciens-mediated transformation, in order to find an association between interrupted gene and the biosynthesis of ochratoxin A. The integration occurred in a gene that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ochratoxigenic fungi; Mycotoxin; Real time PCR; Splicing coactivator protein. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700018 |
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| Carneiro,Monalisa Sampaio; Vieira,Maria Lucia Carneiro. |
| Ao lado dos projetos de seqüenciamento e das análises do cariótipo pelas técnicas de hibridização in situ, o desenvolvimento de mapas genéticos fundamentados em marcadores de DNA tem propiciado consideráveis avanços à genômica de plantas. Esta revisão aborda as premissas básicas utilizadas para o mapeamento genético e suas principais aplicações, especialmente para o melhoramento vegetal. Fundamentos teóricos sobre segregação, recombinação e ligação são considerados e relacionados à construção de mapas genéticos com marcas moleculares. Apresentam-se informações sobre tipos de marcadores, populações de mapeamento, cálculo da freqüência de recombinação, distorções da segregação, estabelecimento dos grupos de ligação e da ordenação dos marcadores. Discute-se,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Mapas de ligação; Marcadores moleculares; QTL; Mapeamento comparativo. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052002000200002 |
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| Vieira,Maria Lucia Carneiro; Santini,Luciane; Diniz,Augusto Lima; Munhoz,Carla de Freitas. |
| Abstract Microsatellites or Single Sequence Repeats (SSRs) are extensively employed in plant genetics studies, using both low and high throughput genotyping approaches. Motivated by the importance of these sequences over the last decades this review aims to address some theoretical aspects of SSRs, including definition, characterization and biological function. The methodologies for the development of SSR loci, genotyping and their applications as molecular markers are also reviewed. Finally, two data surveys are presented. The first was conducted using the main database of Web of Science, prospecting for articles published over the period from 2010 to 2015, resulting in approximately 930 records. The second survey was focused on papers that aimed at SSR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SSR biological function; Genomic distribution; Genotyping approaches; Molecular marker; Practical utility. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300312 |
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| Alves,Adriana Cristina; Quecini,Vera Maria; Vieira,Maria Lucia Carneiro. |
| Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation), screening (reporter gene possibilities and performance) and transformant selection (ipt selective marker) are discussed. Transgene expression and mechanisms underlying (trans)gene inactivation are presented.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant transformation; Agrobiolistics; Electroporation; Reporter genes; Transgenic crops. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000100001 |
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| Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de. |
| A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Population genetics; Plant breeding; Molecular markers. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022 |
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